Fig. 7: Lig3, Lig1, and PARP1 gene silencing suppresses abnormal proliferation, migration, invasion capabilities of NCSC-MYCN | Cell Death & Disease

Fig. 7: Lig3, Lig1, and PARP1 gene silencing suppresses abnormal proliferation, migration, invasion capabilities of NCSC-MYCN

From: Alternative NHEJ pathway proteins as components of MYCN oncogenic activity in human neural crest stem cell differentiation: implications for neuroblastoma initiation

Fig. 7

a A MTS/PMS assay was used to measure cell proliferation. siLig3 cells had more than 72% reduction in cell survival, siLig1 had more than 82% reduction in cell survival, and siPARP1 had more than 67% reduction in survival compared to siCtrl (***p < 0.001,****p < 0.0001). Representative microscopy images (20X) of siCtrl, siLig3, siLig1, and siPARP1 are shown. b and c All three subsets of knockdown cells had significant reduction in migration and invasion rates compared to siCtrl (****p < 0.0001). Bars represent mean ± 2SD. Statistical analyses were performed with ANOVA

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