Fig. 8: Lig3, Lig1, and PARP1 individual gene silencing in NCSC-MYCN cells induced a mature cell phenotype and downregulated NBL markers | Cell Death & Disease

Fig. 8: Lig3, Lig1, and PARP1 individual gene silencing in NCSC-MYCN cells induced a mature cell phenotype and downregulated NBL markers

From: Alternative NHEJ pathway proteins as components of MYCN oncogenic activity in human neural crest stem cell differentiation: implications for neuroblastoma initiation

Fig. 8

a Neurite length was measured using ImageJ. siLig3, siLig1, and siPARP1 cells had 3 to 5 times increased neurite length than compared to controls. (**p < 0.005, ***p < 0.001) b and c Protein expression levels of NBL tumor markers (TH, Phox2b, and TRKB) were determined in siCtrl, siLig3, siLig1, and siPARP1 cells using immunocytochemistry and immunoblotting. Gene silencing of alt-NHEJ components resulted in significantly decreased expression of all three NBL markers tested. Bars represent mean ± 2SD. Statistical analyses were performed with ANOVA

Back to article page