Fig. 1: Impaired in vitro Th1 differentiation of CD4⁺ T-cells from Lmna^−/− mice

Percentage of IFNγ⁺ and IL4⁺ CD4⁺ T-cells isolated from WT or Lmna^−/− mice. a CD4⁺ T-cells from WT/OTII or Lmna^−/−/OTII mice were cocultured with OVA-loaded WT APCs for 4 days. Data are means±SEM of at least three independent experiments analyzed by unpaired Student’s t-test. b CD4⁺ T-cells were stimulated with anti-CD3/CD28 antibodies for 4 days in the presence of IL-2. Data are means±SEM of at least five independent experiments analyzed by unpaired Student’s t-test. c CD4⁺ T-cells were stimulated with anti-CD3/CD28 antibodies for 4 days in the presence of cytokines and antibodies to trigger Th1 or Th2 differentiation in vitro. Data are means±SEM of at least four independent experiments analyzed by unpaired Student’s t-test. d CD4⁺ T-cells from WT/OTII or Lmna^−/−/OTII mice were cocultured with OVA-loaded WT APCs for 4 days in the presence of cytokines and antibodies to trigger Th1 differentiation. Data are means±SEM of three independent experiments analyzed by unpaired Student’s t-test. **P < 0.01; ***P < 0.001