Fig. 2: Impaired in vivo Th1 differentiation of naive CD4⁺ T-cells from Lmna^−/− mice

Percentage of IFNγ⁺ or IL4⁺ CD4⁺ T-cells upon VACV infection. a, b WT and Lmna^−/− mice were intraperitoneally infected with VACV, and after 3 days mesenteric lymph nodes a and peritoneal exudate b were analyzed, (n = 11 WT and 7 Lmna^−/− mice). Data are means±SEM analyzed by unpaired Student’s t-test. c Irradiated CD45.1⁺ WT mice were reconstituted with CD45.2⁺ WT or CD45.2⁺ Lmna^−/− bone marrow and infected intraperitoneally with VACV. After 5 days, spleens and the peritoneal exudate were analyzed by flow cytometry (n = 7 and 8–11 mice from two independent experiments). Data are means±SEM analyzed by unpaired Student’s t-test d Irradiated CD45.1⁺ WT mice were reconstituted with CD45.2⁺ WT/OTII or CD45.2⁺ Lmna^−/−/OTII bone marrow and infected intraperitoneally with VACV-OVA. After 5 days, peritoneal exudate was analyzed (n = 9 and 6 mice from 2 independent experiments). Data are means±SEM analyzed by unpaired Student’s t-test. e Irradiated CD45.1⁺/CD45.2⁺ WT mice were reconstituted with a mix of CD45.1⁺ WT/OTII with CD45.2⁺ Lmna^−/−/OTII bone marrow. Five days after VACV-OVA intradermal infection in the footpad, popliteal lymph nodes were analyzed (n = 9 and 9 mice from 2 independent experiments). Data are means±SEM analyzed by paired Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001