Fig. 3: Compromised in vivo Th1 differentiation of naive CD4⁺ T-cells from Lmna^−/− mice

Percentage of IFNγ⁺ CD4⁺ T-cells. a Recipient CD45.1⁺ WT mice were adoptively transferred with CD45.2⁺ WT/OTII or CD45.2⁺ Lmna^−/−/OTII CD4⁺ T-cells. Five days after intraperitoneal VACV-OVA infection, mesenteric lymph nodes and the peritoneal exudate were analyzed (n = 10 WT and 9 Lmna^−/− mice from 2 independent experiments). Data are means±SEM analyzed by unpaired Student’s t-test. b CD45.1⁺/CD45.2⁺ WT mice were adoptively transferred (AT) with a mix of CD45.1⁺ WT/OTII and CD45.2⁺ Lmna^−/−/OTII CD4⁺ T-cells and infected intraperitoneally with VACV-OVA. After 5 days, peritoneal exudate and mesenteric lymph nodes were analyzed (n = 11 and 11 mice from 2 independent experiments) Data are means±SEM analyzed by paired Student’s t-test. c CD45.1⁺/CD45.2⁺ WT mice were AT as in b, and after 5 days of intradermal infection with VACV-OVA the popliteal lymph nodes were analyzed (n = 6 and 6 mice from 2 independent experiments). Data are means±SEM analyzed by paired Student’s t-test d OVA-loaded LPS-matured BMDCs were AT to WT recipient mice. After 18 h, recipients received intravenous injections of a mix of CD45.1⁺ WT and CD45.2⁺ Lmna^−/−/OTII CD4⁺ naive T-cells. Four days later, the popliteal lymph nodes were analyzed (n = 3 and 3 mice from 1 representative experiment out of 2). Data are means±SEM analyzed by unpaired Student’s t-test e Isolated splenic CD11c⁺ cells were incubated with OVA and LPS or with OVA and papain for 4 h and then AT to WT recipients. Control mice were transferred with CD11c⁺ cells in the absence of OVA (DC-OVA). After 18 h, recipients received intravenous injections of a mix of CD45.1⁺ WT and CD45.2⁺ Lmna^−/− CD4⁺ naive T-cells. Four days later, the popliteal lymph nodes were analyzed. The percentage of IL4⁺ CD4⁺ T-cells was also investigated (n = 4 and 4 mice from 2 independent experiments). Data are means±SEM analyzed by unpaired Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001