Fig. 5: Lmna^−/− CD4⁺ T-cells show impaired in vitro and in vivo T-bet expression

a Percentage of IFNγ⁺ and T-bet⁺ cells after stimulation of CD4⁺ T-cells from WT and Lmna^−/− mice with anti-CD3/CD28 antibodies or PMA plus ionomycin for 4 days in the presence of IL-2 (Data are means±SEM of at least 2 independent experiments analyzed by one-way ANOVA with Bonferroni’s multiple comparison test. b T-bet and lamin A/C expression in WT and Lmna^−/− CD4⁺ T-cells 48 h after stimulation with anti-CD3/CD28 antibodies (n = 12 and 12 mice). Data are means±SEM analyzed by one-way ANOVA with Bonferroni’s multiple comparison test c Percentage of T-bet⁺ cells after stimulation of WT and Lmna^−/− CD4⁺ T-cells with anti-CD3/CD28 for 7 days in the presence of Th1 polarizing antibodies and cytokines (n = 5 and 6 mice from 2 independent experiments). Data are means±SEM analyzed by unpaired Student’s t-test d Percentage of T-bet⁺ CD4⁺ T-cells in peritoneal exudates and mesenteric lymph nodes of WT recipient mice reconstituted with a 1:1 mix of WT and Lmna^−/− bone marrow and intraperitoneally infected with VACV for 6 days (n = 4 and 4 mice from 2 independent experiments). Data are means±SEM analyzed by paired Student’s t-test e RT-qPCR analysis of the indicated genes in WT and Lmna^−/− CD4⁺ T-cells stimulated in vitro with anti-CD3/CD28 antibodies (n = pools of 3 mice). Data are means±SEM from 3 independent experiments analyzed by unpaired Student’s t-test *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001