Fig. 6: Lamin A/C deficiency enhances susceptibility to VACV and Leishmania major

a–c Rag1^−/− mice were inoculated intravenously with naive WT or Lmna^−/− CD4⁺ T-cells and infected with VACV by tail scarification. After 10 days, animals were analyzed to determine a the percentage of IFNγ⁺ CD4⁺ T-cells in the spleen (n = 7 and 5 mice), b the viral titers in the tail (n = 5 and 5 mice), and c the mRNA levels of T-bet (Tbx21), IFNγ, and Gata3 in the gut (n = 7 and 8 mice). d–h WT mice were adoptively transferred with WT or Lmna^−/− bone marrow. Two months after reconstitution, Leishmania major promastigotes were injected intradermally in the ears (n = 8–9 mice). Lesion size d and ear thickness e were quantified at the indicated times. f Parasite burden in ears and draining lymph nodes was measured 3 and 10 weeks after infection. g, h The percentages of g IFNγ⁺ CD4⁺ T-cells and h the CD4⁺ T-cell IFNγ content were quantified in the ears 3 weeks after infection. i–k WT and CD4-CRE Lmna^flox/flox mice were injected intradermally in the ears with Leishmania major promastigotes (n = 9 and 9 mice). i Lesion size at the indicated times. j Parasite burden in the ears and draining lymph nodes at 3 weeks postinfection. k Percentage of IFNγ⁺ CD4⁺ T-cells in the ears. Data analysis in a–c, f–h, k: unpaired Student’s t-test; d–e, i: two-way ANOVA with Bonferroni’s post-hoc multiple comparison test. *P < 0.05; **P < 0.01; ***P < 0.001