Fig. 5 | Cell Death & Disease

Fig. 5

From: Epithelial cell adhesion molecule overexpression regulates epithelial-mesenchymal transition, stemness and metastasis of nasopharyngeal carcinoma cells via the PTEN/AKT/mTOR pathway

Fig. 5

EpCAM activates the PTEN/AKT/mTOR signalling pathway in NPC cells. a, b Western blotting analysis revealed that EpCAM overexpression decreases PTEN expression and increases phosphorylated protein levels of mTOR, AKT, S6K and 4EBP1 (p-mTOR, p-AKT, p-p70S6k and p-4EBP1) in S-18 and 6–10B cells a, whereas knockdown of EpCAM increased PTEN and decreased the protein levels of p-mTOR, p-AKT, p-p70S6k and p-4EBP1 in HONE1 cells b. GAPDH was used as a loading control. c Correlation between EpCAM and PTEN and p4E-BP1 expression in NPC tissues (n = 23). Immunohistochemical staining of representative NPC samples is shown (magnification ×200). Pearson correlation coefficient was calculated on the basis of the relative protein expression levels (IHC scores) of EpCAM, PTEN and p4E-BP1 in NPC samples

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