Fig. 2: IBTKα RNA interference increases spontaneous and fludarabine–induced apoptosis in MEC-1 CLL cells | Cell Death & Disease

Fig. 2: IBTKα RNA interference increases spontaneous and fludarabine–induced apoptosis in MEC-1 CLL cells

From: The expression of inhibitor of bruton’s tyrosine kinase gene is progressively up regulated in the clinical course of chronic lymphocytic leukaemia conferring resistance to apoptosis

Fig. 2

MEC-1 cells were transduced with TurboGFP-shCTRL or TurboGFP-shIBTKα (Sigma-Aldrich), and after 5 days were left untreated (N.T.) a, b or 72 h-treated with Fludarabine at 25 µM c and d or 50 µM e, f. Then, cells were collected, stained with Annexin-V-APC and analysed by flow cytometry. Binding of Annexin-V-APC was measured for GFP-positive cells, as positive control of transduction. A representative experiment of three independent experiments is shown. Statistically significant differences were assessed using Mann–Whitney test (GraphPad Prism 6), as follows: a vs. b p = 0.0043; c vs. d p = 0.0002; e vs. f p = 0.0001

Back to article page