Fig. 4: IBTKα expression inversely correlates with TNFα expression in the CLL progression | Cell Death & Disease

Fig. 4: IBTKα expression inversely correlates with TNFα expression in the CLL progression

From: The expression of inhibitor of bruton’s tyrosine kinase gene is progressively up regulated in the clinical course of chronic lymphocytic leukaemia conferring resistance to apoptosis

Fig. 4

a DeFew cells were transduced with shCTRL or shIBTKα, and after 5 days the total RNA was analysed by RT-qPCR, using RT2 Profiler PCR Array-Human Apoptosis (Qiagen) Values (mean ± SE, n = 3) are shown. Mean of ACTB, B2M and GAPDH expression was used for normalisation. b B-cells were isolated from healthy donors (HD), and representative patients of Binet A (CLL47), B (CLL43) and Binet C (CLL52) stages. Total RNA was analysed for the expression of IBTKα and TNFα by RT-qPCR. β-ACTIN expression was measured for normalisation. Statistical analysis was performed using Mann-Whitney test (GraphPad Prism 6). Mean values (n = 3) ± SE are shown

Back to article page