Fig. 3

Mir-770 could be packed in TNBC derived exosomes and transferred to influence to phenotype of TAMs. a The expression of miR-770 in exosomes that derived from TNBC cells transfected with miR-NC and miR-770. b The ideograph of co-culture system. c MDA-MB-468 and MDA-MB-231 was transiently transfected with FAM labeled miR770 or without transfection (Control) were co-cultured with PMA treated THP-1. Fluorescence microscopy was used to detect the green signals in THP-1 cells. d The relative miR-770 expression in THP-1 cells co-cultured with TNBC cells transfected with miR-NC or miR-770. e THP-1 cells were treated with 150 nM PMA for 24 h and were transfected with miR-NC/770, then 20 ng/ml IL-4 and 20 ng/ml were added for another 24 h. The mRNA of both cells were isolated and the relative mRNA expression of M1 and M2 markers was detected. f THP-1 cells were treated as above, the protein was isolated and iNOS and Arg-1 were detected. g THP-1 cells were treated as above, then the conditioned medium (CM) was collected. The IC50 of both MDA-MB-468 and MDA-MB-231 cells to DOX was detected when cultured with 468 CM-miR-NC/770. Data represent means ± S.D. of at least three independent experiments. *P < 0.05, **P < 0.01