Fig. 5 | Cell Death & Disease

Fig. 5

From: MiR-770 suppresses the chemo-resistance and metastasis of triple negative breast cancer via direct targeting of STMN1

Fig. 5

STMN1 mRNA is a direct target of miR-770. a The three predicted targeting sequence of miR-770 of the STMN1 3′ UTR. b Sequence alignment of the predicted miR-770 seed region in the 3′ UTR of STMN1 from 5 organisms. c qRT-PCR and western blot assay revealed that overexpression of miR-770 level significantly reduced STMN1 expression in MDA-MB-468 and MDA-MB-231 cells. d The diagram illustrates the construction of the luciferase reporter plasmids. The substitutions of nucleotides within the three binding sites were shown and the three constructs named as mut1, mut2, and mut 3 separately (Wt: wild type; Mut: mutant type.). e Luciferase reporter assay in HEK293T cells revealed miR-770 suppressed STMN1 3′ UTR luciferase activity of all wide-type constructs. Firefly luciferase activity was normalized to the internal Renilla luciferase activity. Data represent means ± S.D. of at least three independent experiments. *P < 0.05, **P < 0.01

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