Fig. 5: DRG neurons have an impact on Cx43 and N-cadherin expression in MSCs during osteoblast differentiation | Cell Death & Disease

Fig. 5: DRG neurons have an impact on Cx43 and N-cadherin expression in MSCs during osteoblast differentiation

From: Dorsal root ganglion neurons regulate the transcriptional and translational programs of osteoblast differentiation in a microfluidic platform

Fig. 5

Sensory neurons derived from rat DRG (5 × 104 cells/cm2) and rat bone marrow MSCs (104 cells/cm2) were cocultured in microfluidic devices for 7 days. DRG neurons were maintained in DMEM supplemented with 2% (v/v) B-27 and 1 μM AraC; MSCs were incubated in OIM composed of DMEM-low glucose with 10% (v/v) FBS, 1 × 10−9 M dexamethasone, 10 mM β-glycerophosphate, and 50 μg/mL ascorbic acid. a Subcellular distribution of Cx43 and N-cadherin in MSCs was evaluated at 4 and 7 days of coculture by IF using antibodies directed against Cx43 and N-cadherin coupled to Alexa Fluor® 594 (red), and DAPI (nuclei; blue) under a confocal microscope. Scale bar = 100 μm. b Cx43 and N-cadherin expression in MSCs was analyzed at 4 and 7 days of coculture by WB and depicted as a relative ratio to the respective loading control α-tubulin normalized to the monoculture value on day 4. The results represent three independent experiments.

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