Fig. 3: IGF2BP2 is directly inhibited by miR-181a-5p
From: miR-181a-5p suppresses invasion and migration of HTR-8/SVneo cells by directly targeting IGF2BP2
a Construction of a pGL3-Control luciferase vector containing the full-length IGF2BP2 3ʹ-UTR. b The effects of miR-181a-5p mimic and inhibitor on the luciferase activity of the IGF2BP2 WT 3ʹ-UTR reporter were measured. c The IGF2BP2 mRNA and protein levels were both diminished by miR-181a-5p overexpression in HTR-8/SVneo cells. A representative western blotting image with the molecular weight markers depicted on the left in kDa is shown. d The IGF2BP2 mRNA and protein levels were both elevated upon treatment of the miR-181a-5p inhibitor in HTR-8/SVneo cells. A representative western blotting image with the molecular weight markers depicted on the left in kDa is shown. e IGF2BP2 protein level was assessed by western blotting in the 10 paired severe pre-eclamptic placentas and normal placentas mentioned in Fig. 1a. A representative western blotting image of four paired placentas is shown, and the molecular weight markers are depicted on the left in kDa. IGF2BP2 protein level was statistically analyzed by quantitating the intensity of the IGF2BP2 bands relative to that of the corresponding GAPDH ones. N normal pregnancy, sPE severe pre-eclampsia. The results are expressed as the mean ± SD based on at least three independent experiments. *P < 0.05; **P < 0.01
