Table 2 Primers used in qRT-PCR analysis
From: miR-181a-5p suppresses invasion and migration of HTR-8/SVneo cells by directly targeting IGF2BP2
Genes | Primers | Sequence(5ʹ–3ʹ) |
|---|---|---|
hsa-miR-181a-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTCAC |
PCR-F | GCCGAACATTCAACGCTGTCG | |
PCR-R | GTGCAGGGTCCGAGGT | |
U6 | RT | AACGCTTCACGAATTTGCGT |
PCR-F | CTCGCTTCGGCAGCACA | |
PCR-R | AACGCTTCACGAATTTGCGT | |
IGF2BP2 | PCR-F | GTTCCCGCATCATCACTCTTAT |
PCR-R | GAATCTCGCCAGCTGTTTGA | |
GAPDH | PCR-F | ATGGAAATCCCATCACCATCTT |
PCR-R | CGCCCCACTTGATTTTGG |
