Fig. 2: Effect of PNPO-shRNA on cell proliferation and cell cycle | Cell Death & Disease

Fig. 2: Effect of PNPO-shRNA on cell proliferation and cell cycle

From: Pyridoxine 5′-phosphate oxidase is a novel therapeutic target and regulated by the TGF-β signalling pathway in epithelial ovarian cancer

Fig. 2

SK-OV-3 (a, c, e, f, i, k and m) and OVCAR-3 (b, d, g, h, j, l and n) PNPO-shRNA-expressing cells (shPNPO) are generated. a, b The phase contrast and fluorescent images of shPNPO cells at fifth passage are shown. Approximately 99% cells were GFP-positive. c, d PNPO mRNA expression was lower in shPNPO cells than in negative control (NC) cells detected by quantitative qRT-PCR. e−h PNPO protein expression was decreased in shPNPO cells detected by western blot using a specific antibody against human PNPO. Histograms show the semi-quantitative analysis of the relative optical density of protein bands (each n = 3). i and j A time-course study shows the proliferation of NC and shPNPO cells detected by the WST-1 assay. k and l Cell cycle of NC and shPNPO was determined by flow cytometry. m and n Histograms show the percentage of the cell population in each phase. *P < 0.05; **P < 0.01; n = 3 independent experiments

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