Fig. 3: Effect of PNPO-shRNA on cell migration and invasion | Cell Death & Disease

Fig. 3: Effect of PNPO-shRNA on cell migration and invasion

From: Pyridoxine 5′-phosphate oxidase is a novel therapeutic target and regulated by the TGF-β signalling pathway in epithelial ovarian cancer

Fig. 3

SK-OV-3 PNPO-shRNA-expressing cells (shPNPO) and negative control cells (NC) were used in experiments. a Wound healing assay. Photos of the wound were taken at 0, 24, 48 and 72 h after scratching. Original amplification, ×40. b Line graph shows the quantitative analysis of the wound healing at different time point. c Transwell assay of cell migration. Photos were taken at 48 h after seeding. Original amplification, ×200; scale bar, 50 µm. d Histogram shows the quantitative analysis of migrated cells of c, e Cell invasion assay. Photos were taken at 48 h after seeding. Original amplification, ×200; scale bar, 50 µm. f Histogram shows the quantitative analysis of invaded cells of e. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; n = 3 independent experiments

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