Fig. 5: Simultaneous detection of TGF-β2 and phosphorylated c-MET in glioblastoma | Cell Death & Disease

Fig. 5: Simultaneous detection of TGF-β2 and phosphorylated c-MET in glioblastoma

From: Negative control of the HGF/c-MET pathway by TGF-β: a new look at the regulation of stemness in glioblastoma

Fig. 5

Double immunostaining was performed on formalin-fixed, paraffin-embedded surgical glioblastoma specimens. The quantification of each chromogen was performed separately within three randomly selected different microscopic fields of each specimen. Blood vessels were excluded from the measurement. a–c Representative images show hematoxylin and eosin (H&E) staining (upper panels); p-c-MET immunoreactivity in green ((a, c) middle and lower panels) and TGF-β2 in red ((b, c) middle and lower panels) in tumor regions of three patients. Nuclei are counter-stained with hematoxylin (blue). The scale bars correspond to 50 μm. d Graphical representation of the percentage of glioblastomas high/low for either TGF-β2 or p-c-MET or both

Back to article page