Fig. 6: Correlations among TGF-β, HGF and stem cell markers upon single-cell qRT–PCR analysis using freshly resected glioblastoma tissues | Cell Death & Disease

Fig. 6: Correlations among TGF-β, HGF and stem cell markers upon single-cell qRT–PCR analysis using freshly resected glioblastoma tissues

From: Negative control of the HGF/c-MET pathway by TGF-β: a new look at the regulation of stemness in glioblastoma

Fig. 6

a, b Pairwise complete correlations of TGF-β1 vs. HGF, OCT-4, SOX-2, SOX-4, MUSASHI, PAI-1 (a, and HGF vs. OCT-4, SOX-2, SOX-4, NANOG, MUSASHI, and CD133 (b) mean-centered LOG2Ex values for 437 individual glioblastoma cells from 6 patients are shown. Pearson’s correlation coefficients are depicted after Bonferroni correction for multiple testing. Pearson’s correlation coefficients for pairwise complete observations were computed using the corrplot R package version 0.77 and Bonferroni correction was applied to adjust for multiple hypothesis testing. Scatterplots of significant correlations were plotted using the ggpubr R package version 0.1.2. Extreme Cq values of “999” were excluded and low quality cells that did not express the housekeeping genes ARF-1 were discarded. The primer sequences are provided with Supplementary Table S1

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