Fig. 6: Knockdown of JNK attenuates IBV-induced apoptosis | Cell Death & Disease

Fig. 6: Knockdown of JNK attenuates IBV-induced apoptosis

From: Activation of the c-Jun NH2-terminal kinase pathway by coronavirus infectious bronchitis virus promotes apoptosis independently of c-Jun

Fig. 6

a H1299 cells were transfected with siEGFP or siJNK before being infected with IBV at MOI~2 or being mock infected. Cells were harvested at indicated time points and were subjected to Western blot analysis using the indicated antibodies. Beta-actin was included as loading control. Percentage of PARP cleavage was determined as in Fig. 3b. Relative amount of Bcl2 was determined as the band intensity normalized to β-actin, with the mock-infected sample of siEGFP-transfected cells set as one. The experiment was repeated three times with similar results, and the result of one representative experiment is shown. b Huh-7 cells were transfected and infected as in a. The cells were harvested and were subjected for Western blot analysis as in a. Percentage of PARP cleavage and the relative abundance of Bcl2 was determined as in a. The experiment was repeated three times with similar results, and the result of one representative experiment is shown.

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