Fig. 4: Deltarasin inhibits the binding of GTP to Ras and interaction of KRAS with PDEδ | Cell Death & Disease

Fig. 4: Deltarasin inhibits the binding of GTP to Ras and interaction of KRAS with PDEδ

From: Inhibition of KRAS-dependent lung cancer cell growth by deltarasin: blockage of autophagy increases its cytotoxicity

Fig. 4

. (A) Co-immuoprecipitation of PDEδ and KRAS using PDEδ and monoclonal antibodies for the control and deltarasin treatment group in H358 cell line. (B) KRAS–GTP levels were determined by incubating the protein lysates from A549-treated 5 μM deltarasin for 24 h with glutathione S-transferase (GST)-tagged Ras binding domain (RBD) immobilized on glutathione beads. Percentage of KRAS–GTP bindings were compared with untreated control. (C) Cellular localization of was observed by fluorescence microscopy after 5 μM deltarasin treatment for 24 h in both A549 and H358 cells (green signal: immunofluorescence signal of primary antibody against KRAS; Blue signal: Hoechst staining on cell nucleus. Magnification: ×40; scale bar: 15 μm). The results were expressed as the mean ± SD of three independent experiments, *P < 0.05; ***P < 0.001 vs. control

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