Fig. 3: miR-221-3p enhances lymphatic metastasis in vivo | Cell Death & Disease

Fig. 3: miR-221-3p enhances lymphatic metastasis in vivo

From: RETRACTED ARTICLE: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2

Fig. 3

a Correlation of bioluminescence intensity with cell quantity in SiHa-luc-RFP-221-3p, SiHa-luc-RFP-nc, HeLa-luc-RFP-221-3p, and HeLa-luc-RFP-NC cells in vitro. b In vivo bioluminescence images of lymphatic metastasis. Primary tumour (P) in claw pad of mice and metastases (M). Lymph nodes in popliteal and inguinal regions were measured by detecting bioluminescence signals. The ratio of metastasis-positive lymph nodes was calculated. *p < 0.05. c In situ hybridization of miR-221-3p expression in primary tumors of mice injected with SiHa-luc-RFP-221-3p, SiHa-luc-RFP-nc, HeLa-luc-RFP-221-3p or HeLa-luc-RFP-NC cells. d Primary tumour site and lymph nodes with tumour cells were identified by tumour cell-expressed RFP

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