Fig. 3: PUMA is a direct target of miR-663 | Cell Death & Disease

Fig. 3: PUMA is a direct target of miR-663

From: miR-663 sustains NSCLC by inhibiting mitochondrial outer membrane permeabilization (MOMP) through PUMA/BBC3 and BTG2

Fig. 3

a Predicted PUMA 3′UTR binding site for miR-663. The alignment of the seed region of miR-663 with PUMA 3′ UTR is shown. The sites of target mutagenesis are indicated in red. b, c Immunoblot showing inverse correlation of PUMA and miR-663 expression paralleled by PARP-1 cleavage in NIH-H460 b Calu-1 and H1299 cells c. d Luciferase activity in HeLa cells transfected with PUMA UTR wt or mut in combination with a control or miR-663 mimic. The normalized luciferase activity is indicated—mean ± s.e.m., **p = 0.008. e Downmodulation of PUMA by RNA interference can rescue LNA-663 apoptotic effect in NIH-H460 cells as shown by Cell Titer Glo assay. Mean ± s.d., ***p < 0.001, *p < 0.05

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