Fig. 3: PLK4 affected SK-N-BE(2) cell biological characteristics | Cell Death & Disease

Fig. 3: PLK4 affected SK-N-BE(2) cell biological characteristics

From: Polo-like kinase 4 mediates epithelial–mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway

Fig. 3

a Assessment of the transfected efficiency of PLK4 protein expression after infection in SK-N-BE(2) cells. Cell proliferation comparison by MTT assay (b, P = 0.0475) and colony formation assay (c, P = 0.0065) in sh-control and sh-PLK4 SK-N-BE(2) cells. d SK-N-BE(2) cells were stained with FITC Annexin V and 7-AAD, then percentage of apoptotic cells analyzed by flow cytometry in sh-control and sh-PLK4 SK-N-BE(2) cells (P < 0.001), Q4 and Q2 regions represent the rate of early and late apoptotic cells, respectively. e Cell cycle analysis by flow cytometry in sh-control and sh-PLK4 SK-N-SH cells and the percentage of cells in the G0/G1, S, or G2/M phases of the cell cycle is indicated. f Scratch assay comparing the migration in sh-control and sh-PLK4 SK-N-BE(2) cells (P = 0.0093). g Comparison of the invasion potential of sh-control and sh-PLK4 SK-N-BE(2) cells by counting the number of the cells that invaded through matrigel-coated transwell inserts (P < 0.001). All experiments were repeated at least three times. (*P < 0.05, **P < 0.01, ***P < 0.001)

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