Fig. 4: PLK4 promoted epithelial–mesenchymal transition (EMT) in NB cells

a Morphologic change in SK-N-BE(2) cells when PLK4 was downregulated. b Western blot of EMT-associated markers expression (epithelial marker E-cadherin, mesenchymal markers N-cadherin, vimentin, and Slug) in NB cell lines when PLK4 was downregulated. Immunofluorescence staining for EMT-associated markers (epithelial marker E-cadherin, mesenchymal markers vimentin, Slug) of sh-control and sh-PLK4 in SK-N-SH c and SK-N-BE(2) d cells, respectively. e Western blot of total Akt and phosphorylated Akt in sh-control and sh-PLK4 NB cells