Fig. 6: Downregulation of PLK4 in SK-N-BE(2) cells suppressed tumorigenesis and inhibited metastasis in vivo | Cell Death & Disease

Fig. 6: Downregulation of PLK4 in SK-N-BE(2) cells suppressed tumorigenesis and inhibited metastasis in vivo

From: Polo-like kinase 4 mediates epithelial–mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway

Fig. 6

a Tumors from the nude mice that were transplanted with the same number of sh-control and sh-PLK4 SK-N-BE(2) cells (2.5 × 106 cells per mouse) into the inguen (n = 5 per group), 6 weeks later all the mice were sacrificed then evaluated the primary tumors weight (P = 0.0061) and volume (P = 0.041) for two groups. b PLK4 expression level estimated by western blot and immunohistochemistry in primary tumors of two groups. c Assessment of proliferation and apoptosis expression with immunohistochemistry in primary tumors of two groups. d Immunohistochemistry and western blot results of EMT-associated markers expression estimation in the first mouse primary tumor of two groups. e Confirmation of hepatic metastases by hematoxylin and eosin staining. (*P < 0.05, **P < 0.01) (Scale bar, 1.0 mm)

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