Fig. 1: IL-15 drives increased oxygen consumption, complex I activity, and mitochondrial ROS
From: Glycolysis promotes caspase-3 activation in lipid rafts in T cells
Anti-CD3/CD28-activated T cells were cultured for 3 days in IL-2 or IL-15. a Oxygen consumption rate (OCR) was measured by extracellular flux analysis. O Oligomycin A (inhibitor of ATP synthase), F FCCP (uncoupler of the electron transport chain), R/A Rotenone and Antimycin (inhibitors of complexes I and III, respectively; mean ± S.D. of four replicates within the one experiment shown. The graph is representative of two independent experiments). b Complex I activity was measured in mitochondrial (Mito) and cytosolic (Cyto) fractions of IL-2- or IL-15-cultured T cells (the graph is representative of two independent experiments). c Complex I rates of activity in (b) for mitochondrial fractions, normalized to the rate of activity of complex I in mouse liver mitochondria (unpaired t-test; *p < 0.05; mean ± S.D. of data from two independent experiments). d Mitochondrial ROS were measured by the conversion of mitoboronic acid (MitoB) to mitophenol (MitoP), and the ratio of MitoP/MitoB was measured by liquid chromatography tandem mass spectrometry (paired t-test; *p < 0.05; mean ± S.E.M. of means from three independent experiments)
