Fig. 2: miR-374a reduces A549 and pc-9 cell proliferation, migration, and invasion in vivo and in vitro | Cell Death & Disease

Fig. 2: miR-374a reduces A549 and pc-9 cell proliferation, migration, and invasion in vivo and in vitro

From: RETRACTED ARTICLE: Dual roles of miR-374a by modulated c-Jun respectively targets CCND1-inducing PI3K/AKT signal and PTEN-suppressing Wnt/β-catenin signaling in non-small-cell lung cancer

Fig. 2

The effect of miR-374a on the growth of A549 and pc-9 cells was examined by a MTT assay, b cell cycle analysis, c colony formation assay, and d EdU incorporation assay. Mean ± SD (n = 3). Scale bars, 100 μm. Excised tumors 20 days after implantation. L (left) NC, R (right) miR-374a (n = 5). e, f The effect of miR-374a on A549 and pc-9 cell migration and invasion was measured by transwell assay and Boyden Chamber assays. Mean ± SD (n = 3). Scale bars, 100 μm. g Representative bioluminescence imaging of the lungs. On day 30, mice receiving transplants of indicated cells were killed and lungs were collected and lung metastases were confimed by H&E staining. Scale bars, 200 μm. Arrows indicate nests of malignant cells. Mean ± SD (n = 3). *P < 0.05

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