Fig. 3: Regulation of the G1/S transition by TACC3 | Cell Death & Disease

Fig. 3: Regulation of the G1/S transition by TACC3

From: TACC3 transcriptionally upregulates E2F1 to promote cell growth and confer sensitivity to cisplatin in bladder cancer

Fig. 3

a, b EJ or 5637 cells were transfected with NC or TACC3 siRNA for 48 h and were then stained with propidium iodide and analyzed by flow cytometry (n = 3). c Ectopic expression of TACC3 in both HT1197 and TCCSUP cells decreased the percentage of cells in G1 phase in the presence of nocodazole (100 ng/ml) for 16 h (n = 3). d Repression of TACC3 inhibited DNA synthesis, which led to a decrease in the number of cells in S phase as shown by BrdU incorporation assay. e Overexpression of TACC3 promoted DNA synthesis, which led to an increase in the number of cells in S phase as shown by BrdU incorporation assay. Error bars represent mean ± SEM. **p < 0.01, ***p < 0.001

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