Fig. 5: Elevated levels of TACC3 or E2F1 render cells more sensitive to cisplatin | Cell Death & Disease

Fig. 5: Elevated levels of TACC3 or E2F1 render cells more sensitive to cisplatin

From: TACC3 transcriptionally upregulates E2F1 to promote cell growth and confer sensitivity to cisplatin in bladder cancer

Fig. 5

a, b Graphs show an inverse correlation between the IC50 of cisplatin and TACC3 (r = −0.35) or E2F1 (r = −0.54) mRNA levels in bladder cancer cell lines. c Western blot of transcriptionally overexpressed TACC3 or E2F1. d Cells that expressed high levels of TACC3, E2F1, or control vector were exposed to various concentrations of cisplatin for 96 h. Cell viability was measured by MTT assay. ***p < 0.001 determined by one-way ANOVA. e–g QPCR analysis of apoptosis-related genes including Bik, Bim, and Bcl-2 in bladder cancer cell lines with exogenous expression of TACC3 or E2F1 after exposure to a high dose of cisplatin (10 µg/ml). Error bars represented mean ± SEM. **p < 0.01, ***p < 0.001 in one-way ANOVA

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