Fig. 2: Time course analysis of LC3-II accumulation in INS-1E cells reveal that cytokines block autophagy flux | Cell Death & Disease

Fig. 2: Time course analysis of LC3-II accumulation in INS-1E cells reveal that cytokines block autophagy flux

From: Dysfunctional autophagy following exposure to pro-inflammatory cytokines contributes to pancreatic β-cell apoptosis

Fig. 2

Representative Western blot and quantification of LC3I/LC3II and p62/tubulin from INS-1E cells pre-treated or not (ctrl) with IL-1β + IFNγ for 6 h and treated with chloroquine (CQ) a or torin1 b for the indicated time. Data are mean ± SEM of 4–6 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. t0; #P < 0.05 ##P < 0.01 vs. ctrl as determined by two-way ANOVA with ad-hoc post t-test with Sidak’s correction for multiple comparisons

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