Fig. 5: Cytokines have no major impact on mitophagy | Cell Death & Disease

Fig. 5: Cytokines have no major impact on mitophagy

From: Dysfunctional autophagy following exposure to pro-inflammatory cytokines contributes to pancreatic β-cell apoptosis

Fig. 5

a, b Time-course Western blot analyses of chloroquine (CQ) a or torin1 b effect on Parkin, mitofusin2, P-DRP1, DRP-1, and tubulin levels in INS-1E cells pre-treated or not (ctrl) with IL-1β + IFNγ for 6 h. All quantifications are mean ± SEM of 4–5 independent experiments.*P < 0.05, **P < 0.01, ***P < 0.001 vs. t0; #P < 0.05; ##P < 0.01; ###P < 0.001 vs. ctrl as determined by two-way ANOVA with ad-hoc post t-test with Sidak’s correction for multiple comparisons. c Live INS-1E cell imaging of Mitochondrial (white) and RFP-LC3 (red) in control (ctrl) and after a 6 h treatment with IL-1β + IFNγ. Images are representative of five independent experiments

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