Fig. 6: RA suppressed Smad3 phosphorylation and nuclear translocation through activating AMPK in vitro | Cell Death & Disease

Fig. 6: RA suppressed Smad3 phosphorylation and nuclear translocation through activating AMPK in vitro

From: Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKα/Smad3 signaling

Fig. 6

a, b Representative western blots and statistical results. CFs were pretreated with shAmpkα2 or shRNA for 4 h at a MOI of 50, incubated with RA for 30 min and then stimulated with TGF-β (10 ng/ml) for additional 1 h (n = 6). c Characteristic immunofluorescence images of T-Smad3 distribution (n = 6). CFs were pretreated with RA for 30 min and then stimulated with TGF-β (10 ng/ml) for additional 1 h. Green represented T-Smad3, nuclei was stained with DAPI (blue). Values represent the mean ± SEM of three independent experiments. *P < 0.05 vs. the corresponding PBS-treated CFs within shRNA group, #P < 0.05 vs. TGF-β-treated CFs within shRNA group, &P < 0.05 vs. TGF-β-treated CFs in shAmpkα2 group

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