Fig. 2: TMEM170B suppressed breast cancer cell proliferation | Cell Death & Disease

Fig. 2: TMEM170B suppressed breast cancer cell proliferation

From: Transmembrane protein 170B is a novel breast tumorigenesis suppressor gene that inhibits the Wnt/β-catenin pathway

Fig. 2

a The TMEM170B expression in breast cancer cell lines was detected by real-time PCR and immunoblotting. b Immunocytochemical staining analysis of the cell membrane localization of TMEM170B in MCF7 and MDA-MB-231 cells. c The subcellular localization of TMEM170B with Na,K-ATPase (alpha 1), a membrane marker of plasma membrane, was analyzed by immunostaining (upper: scale bar, 20 μm; lower: scale bar, 5 μm). d, e Relative expression of miR-27a in the indicated MCF7 cell clones (d) and MDA-MB-231 cell clones (e). Cell proliferation (f) and soft agar assays (g) were performed on MCF7 cells with TMEM170B knockdown. Cell proliferation (h) and soft agar assays (i) were performed on MDA-MB-231 cells with overexpressing TMEM170B. Values are shown as mean ± S.E.M. from three independent experiments repeated in triplicates. *P < 0.05 vs. CTRL, **P < 0.01 vs. CTRL

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