Fig. 1: Expression of TLR4 in hNSC
From: Toll-like receptor 4 modulation influences human neural stem cell proliferation and differentiation
a Flow cytometric analysis of TLR4, MD-2 (top hystograms), and CD14 (bottom hystograms) surface expression on neural stem cells (left) and monocytes (right); Relative MFI quantification (bar graphs) of TLR4, MD-2, and CD14 surface expression in neural stem cells (left) and monocytes (right). Data are representative of two independent experiments. b Real-time PCR of TLR4 gene in hNSC untreated or treated for 24 and 48 h with LPS 100 nM or FP7 10 μM. c Western blot analysis of TLR4 expression in THP-1 (positive control) cells and hNSC untreated or long-term treated with LPS 10 nM, LPS 100 nM, FP7 1 μM, or FP7 10 μM. d Real-time PCR of COX2 and mPGES-1 genes in hNSC untreated or treated for 24 and 48 h with LPS 100 nM or FP7 10 μM
