Fig. 4: Mitochondrial pattern and differentiation markers in dissociated hNSC neurospheres after long-term TLR4 modulation
From: Toll-like receptor 4 modulation influences human neural stem cell proliferation and differentiation
a Mitotracker staining and immunofluorescence analysis of expression of βtubIII, Casp1, Ki67 and Casp3 markers of hNSC dissociated neurospheres after long-term treatment with LPS 10 nM, LPS 100 nM, FP7 1 μM, FP7 10 μM, LPS 100 nM+ FP7 10 μM and AbTLR. Scale bars: 20–75 μm. b–d charts showing the quantitative analysis of the percentage of βtubIII+ b, Ki67+ c and Casp3+ d cells over the total DAPI+ nuclei. Statistical significance is indicated. Values are means ± eSEM. Scatter distribution plot representing densitometric analysis of Casp1 relative fluorescence intensity (n ≥ 38 cells). Statistical analysis is indicated
