Fig. 5: Effects of TLR4 modulation on early differentiation of hNSC
From: Toll-like receptor 4 modulation influences human neural stem cell proliferation and differentiation
a Immunofluorescence analysis of expression of βtubIII, GFAP, MAP2, Ki67, and GalC markers in hNSC differentiated at 10 div after treatment from 0 div with LPS 10 nM, LPS 100 nM, FP7 1 μM, FP7 10 μM, LPS 100 nM+ FP7 10 μM, and AbTLR block. Scale bars: 50 μm. b–h Charts showing the quantitative analysis of the percentage of βtubIII+ (b), GFAP+ (c), MAP2+ (d), Ki67+ (e), double-stained MAP2/KI67+ (f), GalC+ (g), and Casp3+ (h) cells over the total DAPI+ nuclei. Statistical significance is indicated for charts (b, f, g). Values are means ± SEM
