Fig. 8: hNSC retain TLR4 expression in vivo
From: Toll-like receptor 4 modulation influences human neural stem cell proliferation and differentiation
a Left panel. Schematic of a coronal section of the spinal cord showing hNSCs (green dots) transplanted in anterior horns (AH) and migrating to surrounding regions. Expression of TLR4 (red) in L3–L4 tract of wild-type rats (upper panel, right), SOD1 not-transplanted controls (mid panel) and transplanted SOD1 rats (lower panel) at 15 and 40 DAT. Colocalization of TLR4 (red) with human-specific antigen (huN, green) or huNestin in transplanted hNSC is shown in the z-stacks (arrows, in lower panel). Scale bars: Upper, mid and lower panel: 40 μm, z-stacks: 10 μm. b Right panel. Schematic of a brain coronal section showing hNSCs (green dots) injected in the lateral ventricle (LV) and migrating into the corpus callosum (CC). Expression of TLR4 (red) in the mouse brain upper panel (right), and in hNSCs (arrows) at 10 (mid panel, left) and 20 (mid panel right) DAT. Colocalization of TLR4 with huN or huNestin is shown in the z-stacks (lower panels). Scale bars: Upper, mid and lower panel: 40 μm, z-stacks: 10 μm
