Fig. 2: Roy-Bz selectively translocates PKCδ to the perinuclear region in human tumor cells

HCT116 cells transfected with pEGFP-N1-PKCα, pEGFP-N1-PKCδ, or pEGFP-N1-PKCε were treated with PMA, Roy-Bz, or vehicle for 1 h; PKC localization was determined by confocal microscopy. a Images are representative of three independent experiments; scale bar = 10 µm and magnification = ×100. b Quantification of cells with PKC translocation; data are mean ± SEM of three independent experiments; values significantly different are indicated (***p < 0.001), unpaired Student’s t-test