Fig. 5: Decreased PKM2 promoted autophagy of BXPC-3 cells in hypoglucose by upregulating AMPKα1 expression
From: The responsively decreased PKM2 facilitates the survival of pancreatic cancer cells in hypoglucose
a The expression level of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR after transfection with pCDNA-PKM2 in BXPC-3-LG. b The expression of PKM2, AMPKα1, and autophagy-related protein was determined by western blot. c Immunofluorescence revealed the autophagic level of pCDNA-PKM2-transfected cells in hypoglucose condition. d The expression level of PKM2, AMPKα1, and Beclin1 was tested by qRT-PCR after transfection with siPKM2 in BXPC-3-LG. e The expression of PKM2, AMPKα1, and autophagy-related protein was determined by western blot after transfection with siPKM2 in BXPC-3-LG. f Immunofluorescence revealed the autophagic level of siPKM2-transfected cells in hypoglucose condition. The autophagosome was indicated with the arrow (*P < 0.05; **P < 0.01)
