Fig. 4: AEAC increases the effects of doxorubicin on the viability of C6 glioma and B16 melanoma cells | Cell Death & Disease

Fig. 4: AEAC increases the effects of doxorubicin on the viability of C6 glioma and B16 melanoma cells

From: Sensitizing tumor cells to conventional drugs: HSP70 chaperone inhibitors, their selection and application in cancer models

Fig. 4

C6 glioma cells (left panel) and B16 melanoma cells (right panel) were seeded in the wells of 96-well plates and incubated with AEAC alone or in combination with doxorubicin for 24 h. Cell media from the wells was then used to measure LDH activity using the Cytotox96 kit a and the cells in the wells were used for the MTT assay b (each single concentration of drugs, or combination of drug, was used in triplicates). Data is presented as the means ± standard error of the mean (SEM). A statistical difference was determined by a value of p < 0.05; c Proliferation rates of C6 or B16 cells incubated with AEAC and doxorubicin alone or in combination. The data are shown as cell index graphs. d Cells were incubated with AEAC alone or in combination with doxorubicin, and cells with apoptotic nuclei were calculated. All experiments were made in triplicates. Statistical significance is indicated as * p < 0,0.1

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