Fig. 2: Candidate ZAK-induced EMT transcriptional factors
From: Mixed lineage kinase ZAK promotes epithelial–mesenchymal transition in cancer progression
a Relative luciferase activities of 23 promoter luciferase reporter constructs transiently co-transfected with ZAK or vector control, along with TK-renilla luciferase vector (pRL-TK, transfection control) into HEK293T cells. Shown on the Y-axis is fold changes of promoter luciferase activities induced by ZAK vs. vector control. Error bars denote SD from experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 (One-way ANOVA test with Tukey’s post hoc test). b Scatter plots obtained from cBioPortal TCGA database show correlation of ZAK mRNA expression with ZEB1 and SNAI1 mRNA expression (accessed in August 2016). Pearson’s correlation coefficients R and P-values are indicated. c ZAK-induced alterations of ZEB1 and downstream genes in HMLE, PC3 and HEK293T cells determined by real time PCR (left) and Western Blot (right) analysis. Error bars denote SD from triplicate. d shRNA silencing of ZEB1 gene and resultant reversal of EMT gene expression in PC3-ZAK and 293T-ZAK cells. Changes in the expression of ZEB1 and EMT markers were determined by real time PCR. Error bars denote SD from triplicate. These experiments were repeated twice with similar results. Additional data related to Fig. 2 are in Supplementary Figure S2
