Fig. 3: RNA-seq analyses confirmed the vascular cell identity of the iECs | Cell Death & Disease

Fig. 3: RNA-seq analyses confirmed the vascular cell identity of the iECs

From: FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cells

Fig. 3

Heat map of marker gene panels for endothelium (a), pluripotency (b), the three germ layers (c, d, e), PKC-related genes (f according to KEGG), FLI-related genes (g according to Ingenuity Pathway Analysis) and small nuclear RNA (h) in hESCs (D0), iECs (D1, D2 and D3) and EPCs. Columns represent genes, and rows are samples. Column Z-score transformation was performed on log2 values for each gene with blue denoting a lower and red denoting a higher expression level than the average expression level. Hierarchical clustering of genes and samples are based on average linkage and correlation distance. i Dynamic gene expression of representative spatio-temporally regulated genes during hESC-EC differentiation. The mean minimal cycle threshold values were calculated from triplicate reactions

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