Fig. 3: DTX1 binds c-FLIP L and promotes c-FLIP L downregulation
From: c-FLIP is a target of the E3 ligase deltex1 in gastric cancer
a DTX1 promotes c-FLIP degradation. 293T cells were transfected with c-FLIPL-Flag (200 ng) or c-FLIPS-Flag (2 ng) with increasing amounts of DTX1-Myc. The levels of DTX1, c-FLIPL and c-FLIPS were determined 24 h after transfection. b The caspase-like domain of c-FLIP binds DTX1. DTX1-Myc, c-FLIPL, c-FLIP-p43, c-FLIPS and the caspase-like domain (CLD) were transfected into 293T cells as indicated. Cell lysates were prepared 24 h later and were immunoprecipitated by anti-Myc. The presence of DTX1 and different forms of c-FLIP in the precipitates and lysates were determined by anti-Myc and anti-Flag. c The p20 region in the CLD of c-FLIP binds DTX1. DTX1-Myc, c-FLIPL, and c-FLIP-p20 were transfected into 293T cells as indicated. The levels of DTX1 and different forms of c-FLIP in lysates and anti-Myc immunoprecipitates were determined by anti-Myc and anti-Flag. Experiments (a–c) were independently repeated three times with similar results
