Fig. 6: Geldanamycin enhances TRAIL-induced apoptosis through DTX1-mediated c-FLIP L degradation
From: c-FLIP is a target of the E3 ligase deltex1 in gastric cancer
a, b DTX1 knockdown reduces GA-enhanced TRAIL sensitivity. Control (pLL3.7) and DTX1-knockdown (shDTX1) AGS cells (a) or SNU-16 cells (b) were incubated with GA overnight followed by TRAIL treatment for 5 h. Apoptosis was determined by Annexin V/PI staining. Values are means ± standard deviations of an experiment with triplicate (a) or three independent experiments (b). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Experiment (a) was repeated three times with similar results. c DTX1-knockdown attenuates GA-induced c-FLIPL degradation. Control (pLL3.7) and DTX1-knockdown (shDTX1) AGS cells were treated with GA overnight. The levels of DTX1, c-FLIPL, and c-FLIPS were determined. Experiments (c) was independently repeated three times with similar results
