Fig. 7: DTX1-overexpression enhances FasL-induced, TRAIL-induced and TNF-α-induced apoptosis in Jurkat cells
From: c-FLIP is a target of the E3 ligase deltex1 in gastric cancer
a Reduced c-FLIP levels in DTX1-overexpressing Jurkat cells. The levels of c-FLIPL, c-FLIPS, and DTX1(Myc) in control (YFP) and DTX1-expressing Jurkat cells were determined. b–d Control (YFP) and DTX1-expressing Jurkat cells were treated with FasL b, TRAIL c, or TNF-α d at the indicated concentrations. The extent of apoptosis was analyzed 6 h later by flow cytometry with Annexin V staining. Data are mean ± SD of a triplicate experiment. Experiments were independently repeated three times with similar results. ***P ≤ 0.001. The experiment was independently repeated twice with comparable results
