Fig. 1: Dysregulated expression of HBP1 and miR-29c in NPC tissues or cell lines

a In situ hybrization and immunohistochemistry assays analysis for miR-29c and HBP1 expression in NPC tissues. Scale bars: upper, 50 μm; lower, 20 μm. Right: Statistical analysis for HBP1 and miR-29c expression between peri-tumor and NPC tissues. HBP1, p < 0.0001, miR-29c, p < 0.0001. b Immunofluorescence analysis for HBP1 (green) and in situ hybrization analysis for miR-29c (brown) in NP69, HK1, HNE1, and CNE2 cells. Scale bars, 50 μm. c RT-qPCR analysis for HBP1 and miR-29c levels in NP69, HNE1, HNE2, HK1, CNE2, and C666–1 cell lines. GAPDH was used as an internal control for mRNA and U6 was used as an internal control for miRNA