Fig. 3: HBP1 is negatively regulated by miR-29c | Cell Death & Disease

Fig. 3: HBP1 is negatively regulated by miR-29c

From: HMG-box transcription factor 1: a positive regulator of the G1/S transition through the Cyclin-CDK-CDKI molecular network in nasopharyngeal carcinoma

Fig. 3

a and b RT-qPCR and western blotting assays for detecting HBP1 mRNA and protein expression levels in HNE1 and CNE2 cell lines after transfection with pre-miR-29c. Coll1a is regarded as a positive target of miR-29c. c Upper: Prediction of binding sites between hsa-miR-29c and the HBP1 3’UTR (Untranslated Region) by Target Scan system. Lower: Sequencing results of miR-29c seed sequences which binding to HBP1 3’UTR wild type (WT) and HBP1 3’UTR mutant. d Luciferase activity of 293T cells transfected with luciferase constructs containing HBP1 3’ UTR wild-type or mutation seed sequences of miR-29c-targeted, p = 0.009. e MiR-29c is inversely correlated with HBP1 expression in NPC tissues from GSE12452 data set (Pearson R = −0.3850, p = 0.0325). f MiR-29c is correlated negatively with HBP1 expression in the NPC cell lines (Pearson R = −0.8393, p = 0.0366). Basal miR-29c levels were normalized to U6 and basal HBP1 levels were normalized to GAPDH in NPC cell lines (Fig. 1c), Log2 transformed

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