Fig. 4: HBP1 facilitates NPC cell proliferation in vitro | Cell Death & Disease

Fig. 4: HBP1 facilitates NPC cell proliferation in vitro

From: HMG-box transcription factor 1: a positive regulator of the G1/S transition through the Cyclin-CDK-CDKI molecular network in nasopharyngeal carcinoma

Fig. 4

a Upper: RT-qPCR analysis for miR-29c miRNA levels in HK1, HNE1, and CNE2 cell lines after transfection with miR-29c mimics or NC mimics. Lower: RT-qPCR analysis for HBP1 mRNA levels in HNE1 and CNE2 cell lines after transfection with siHBP1 and siNC. b Upper: CCK-8 assays analysis for HK1, HNE1, and CNE2 cells transfected with miR-29c mimics or NC mimics. Lower: CCK-8 assays analysis for HK1, HNE1, and CNE2 cells transfected with siHBP1 or siNC. c Ki67 cell proliferation assay for HK1, HNE1 and CNE2 cell lines after transfection with pre-miR-29c or vector, siHBP1 or siNC. The grouped graphs are the semiquantitation for Ki67 protein. t test analyses. ***p < 0.001. Scale bars, 50 μm. d EDU assay for HK1, HNE1, and CNE2 cell lines after transfection with pre-miR-29c or vector, siHBP1 or siNC. The grouped graphs are analyzed for the cell proliferation rate. t test analyses. ***p < 0.001; **p < 0.01. The cell proliferation rate was normalized with the ratio for the EDU-stained cells to the nucleus-stained cells. Scale bars, 100 μm

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