Fig. 2: Human colorectal cancer-derived mesenchymal stem cells promote the migration and invasion of SW48 colorectal cancer in vitro, and mediate epithelial-to-mesenchymal transition (EMT) in colorectal cancer. | Cell Death & Disease

Fig. 2: Human colorectal cancer-derived mesenchymal stem cells promote the migration and invasion of SW48 colorectal cancer in vitro, and mediate epithelial-to-mesenchymal transition (EMT) in colorectal cancer.

From: Human colorectal cancer-derived mesenchymal stem cells promote colorectal cancer progression through IL-6/JAK2/STAT3 signaling

Fig. 2

a Wound-healing closure assays of SW48 colorectal cancer cells were performed in the presence or absence of CC-MSC conditioned medium (CC-MSC-CM) (magnification, ×50; scale bar: 500 μm). b Chemotaxis assays of SW48 cells with CC-MSCs-CM in the lower chamber (×100). c Invasion assays of SW48 colorectal cancer cells were also carried out in the presence or absence of CC-MSCs-CM (×100). The addition of the CC-MSC-CM enhanced both the migration and invasion of the SW48 cells. *P < 0.05 compared with mock treatment. d SW48 cells were cultivated with CC-MSC-CM for different times, and several markers associated with the EMT process, such as E-cadherin, vimentin, and SLUG, were detected by western blotting analysis. F12 served as the control. e SW48 cells in the presence or absence of CC-MSC-CM were photographed using a phase contrast microscope. A mesenchymal phenotype in SW48 cells was viewed in the presence of CC-MSC-CM and compared with the control group (magnification, ×50; scale bar: 500 μm)

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